Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Dihydrofolate Reductase ELISA Kit

DHFR Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690864
  • Target See all Dihydrofolate Reductase (DHFR) ELISA Kits
    Dihydrofolate Reductase (DHFR)
    Reactivity
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human DHFR ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human DHFR.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling DHFR ELISA Kit
    Top Product
    Discover our top product DHFR ELISA Kit
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all Dihydrofolate Reductase (DHFR) ELISA Kits
    Dihydrofolate Reductase (DHFR)
    Alternative Name
    DHFR (DHFR Products)
    Synonyms
    DHFR ELISA Kit, F10_orf160 ELISA Kit, ECK0049 ELISA Kit, JW0047 ELISA Kit, tmrA ELISA Kit, cb595 ELISA Kit, zgc:86637 ELISA Kit, DDBDRAFT_0187132 ELISA Kit, DDBDRAFT_0230113 ELISA Kit, DDB_0187132 ELISA Kit, DDB_0230113 ELISA Kit, CG14887 ELISA Kit, Dmel\\CG14887 ELISA Kit, dhfr ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, 8430436I03Rik ELISA Kit, AA607882 ELISA Kit, AI662710 ELISA Kit, AW555094 ELISA Kit, Dhfr1 ELISA Kit, confers resistance to sulfonamides ELISA Kit, dihydrofolate reductase ELISA Kit, dihydrofolate reductase DhfR ELISA Kit, Dihydrofolate reductase ELISA Kit, dhfr ELISA Kit, DHFR ELISA Kit, dhfR ELISA Kit, folA ELISA Kit, dfrA ELISA Kit, LOC707382 ELISA Kit, CNJ01940 ELISA Kit, DDB_G0286755 ELISA Kit, Dhfr ELISA Kit, Mesil_0096 ELISA Kit, Arch_0408 ELISA Kit, Fbal_3367 ELISA Kit, Ilyop_0482 ELISA Kit, Lbys_2007 ELISA Kit, Riean_0733 ELISA Kit, Mrub_1855 ELISA Kit, Poras_0766 ELISA Kit, Halhy_3552 ELISA Kit, Mesop_3614 ELISA Kit, LOC609048 ELISA Kit
    Gene ID
    1719
    UniProt
    P00374
    Pathways
    Mitotic G1-G1/S Phases
You are here:
Support