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C1QBP ELISA Kit

C1QBP Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690886
  • Target See all C1QBP ELISA Kits
    C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
    Reactivity
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human HABP1/C1qBP ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human HABP1.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all C1QBP ELISA Kits
    C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
    Alternative Name
    HABP1 (C1qBP (C1QBP Products)
    Synonyms
    SF2 ELISA Kit, X38k ELISA Kit, gC1qR ELISA Kit, C1QBP ELISA Kit, GC1QBP ELISA Kit, HABP1 ELISA Kit, SF2p32 ELISA Kit, gC1Q-R ELISA Kit, p32 ELISA Kit, AA407365 ELISA Kit, AA986492 ELISA Kit, D11Wsu182e ELISA Kit, P32 ELISA Kit, gC1qBP ELISA Kit, Habp1 ELISA Kit, complement C1q binding protein ELISA Kit, complement component 1, q subcomponent binding protein S homeolog ELISA Kit, complement component 1, q subcomponent binding protein ELISA Kit, C1QBP ELISA Kit, c1qbp.S ELISA Kit, LOC100227158 ELISA Kit, C1qbp ELISA Kit
    Gene ID
    708
    UniProt
    Q07021
    Pathways
    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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