C1QBP ELISA Kit
C1QBP
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all C1QBP ELISA Kits
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C1QBP
(Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human HABP1/C1qBP ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Human HABP1.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all C1QBP ELISA Kits
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C1QBP
(Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
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Alternative Name
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HABP1 (C1qBP (C1QBP Products)
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Synonyms
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SF2 ELISA Kit, X38k ELISA Kit, gC1qR ELISA Kit, C1QBP ELISA Kit, GC1QBP ELISA Kit, HABP1 ELISA Kit, SF2p32 ELISA Kit, gC1Q-R ELISA Kit, p32 ELISA Kit, AA407365 ELISA Kit, AA986492 ELISA Kit, D11Wsu182e ELISA Kit, P32 ELISA Kit, gC1qBP ELISA Kit, Habp1 ELISA Kit, complement C1q binding protein ELISA Kit, complement component 1, q subcomponent binding protein S homeolog ELISA Kit, complement component 1, q subcomponent binding protein ELISA Kit, C1QBP ELISA Kit, c1qbp.S ELISA Kit, LOC100227158 ELISA Kit, C1qbp ELISA Kit
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Gene ID
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708
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UniProt
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Q07021
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Pathways
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Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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