NEDD8 ELISA Kit (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) ELISA Kit
NEDD8 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant
Catalog No. ABIN5690916
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- Detection Method
- Method Type
- Sandwich ELISA
- Human NEDD8 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- This ELISA antibody pair recognizes Human NEDD8.
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- For Research Use only
- 4 °C
- Expiry Date
- 6 months
- Alternative Name
- NEDD8 (NEDD8 ELISA Kit Abstract)
- NEDD-8, Rub1, CG10679, Dmel\\CG10679, NEDD8, dNEDD8, nedd8, ubiquitin, si:zc14a17.5, GB17785, DDBDRAFT_0218116, DDBDRAFT_0238041, DDB_0218116, DDB_0238041, nedd8.S, neural precursor cell expressed, developmentally down-regulated 8, neural precursor cell expressed, developmentally down-regulated gene 8, CG10679 gene product from transcript CG10679-RB, Nedd8, ubiquitin-like protein Nedd8, neural precursor cell expressed, developmentally down-regulated 8 L homeolog, NEDD8, Nedd8, nedd8, nedd8.L
- Gene ID
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