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NEDD8 ELISA Kit

NEDD8 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690916
  • Target See all NEDD8 ELISA Kits
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Reactivity
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human NEDD8 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human NEDD8.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product NEDD8 ELISA Kit
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all NEDD8 ELISA Kits
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Alternative Name
    NEDD8 (NEDD8 Products)
    Synonyms
    NEDD-8 ELISA Kit, Rub1 ELISA Kit, CG10679 ELISA Kit, Dmel\\CG10679 ELISA Kit, NEDD8 ELISA Kit, dNEDD8 ELISA Kit, nedd8 ELISA Kit, ubiquitin ELISA Kit, si:zc14a17.5 ELISA Kit, GB17785 ELISA Kit, DDBDRAFT_0218116 ELISA Kit, DDBDRAFT_0238041 ELISA Kit, DDB_0218116 ELISA Kit, DDB_0238041 ELISA Kit, nedd8.S ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 8 ELISA Kit, CG10679 gene product from transcript CG10679-RB ELISA Kit, Nedd8 ELISA Kit, ubiquitin-like protein Nedd8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 L homeolog ELISA Kit, NEDD8 ELISA Kit, Nedd8 ELISA Kit, nedd8 ELISA Kit, nedd8.L ELISA Kit
    Gene ID
    4738
    UniProt
    Q15843
    Pathways
    Ubiquitin Proteasome Pathway
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