BCAR1 ELISA Kit

Catalog No. ABIN5690924

Product Details BCAR1 ELISA Kit

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human p130Cas/BCAR1/CAS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human p130Cas.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: p130Cas (BCAR1/CAS) (BCAR1 Products)

Synonyms: CAS ELISA Kit, CAS1 ELISA Kit, CASS1 ELISA Kit, CRKAS ELISA Kit, P130Cas ELISA Kit, AI385681 ELISA Kit, Cas ELISA Kit, Crkas ELISA Kit, P130CAS ELISA Kit, MGC68466 ELISA Kit, cas ELISA Kit, p130cas ELISA Kit, BCAR1 ELISA Kit, fc17f11 ELISA Kit, wu:fc17f11 ELISA Kit, zgc:175192 ELISA Kit, BCAR1, Cas family scaffolding protein ELISA Kit, breast cancer anti-estrogen resistance 1 ELISA Kit, breast cancer anti-estrogen resistance 1 L homeolog ELISA Kit, breast cancer anti-estrogen resistance protein 1 ELISA Kit, BCAR1 ELISA Kit, Bcar1 ELISA Kit, bcar1.L ELISA Kit, bcar1 ELISA Kit, LOC100435586 ELISA Kit

Gene ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling