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PGPEP1 ELISA Kit

PGPEP1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690932
  • Target See all PGPEP1 ELISA Kits
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human PGPEP-1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human PGPEP-1.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all PGPEP1 ELISA Kits
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Alternative Name
    PGPEP-1 (PGPEP1 Products)
    Synonyms
    PGP ELISA Kit, PGP-I ELISA Kit, PGPI ELISA Kit, Pcp ELISA Kit, 2810003H13Rik ELISA Kit, Gm19458 ELISA Kit, PAP-I ELISA Kit, PAPI ELISA Kit, pyroglutamyl-peptidase I ELISA Kit, Pyroglutamyl-peptidase I ELISA Kit, Fnod_0998 ELISA Kit, Tlet_1256 ELISA Kit, Franean1_3440 ELISA Kit, Sked_37410 ELISA Kit, Arch_1014 ELISA Kit, Olsu_1567 ELISA Kit, Bcell_2984 ELISA Kit, Deima_2337 ELISA Kit, Spico_0458 ELISA Kit, Poras_1572 ELISA Kit, PGPEP1 ELISA Kit, Pgpep1 ELISA Kit
    Gene ID
    54858
    UniProt
    Q9NXJ5
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