PLXNB1 ELISA Kit

Catalog No. ABIN5690937

Product Details PLXNB1 ELISA Kit

Target: PLXNB1 (Plexin B1 (PLXNB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Plexin-B1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Plexin-B1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for PLXNB1

Target: PLXNB1 (Plexin B1 (PLXNB1))

Alternative Name: Plexin-B1 (PLXNB1 Products)

Synonyms: im:7054247 ELISA Kit, wu:fb83a09 ELISA Kit, si:ch211-220i18.5 ELISA Kit, 2900002G15Rik ELISA Kit, AU042020 ELISA Kit, mKIAA0407 ELISA Kit, PLEXIN-B1 ELISA Kit, PLXN5 ELISA Kit, SEP ELISA Kit, SET domain containing 5 ELISA Kit, plexin B1 ELISA Kit, plexin b1a ELISA Kit, SETD5 ELISA Kit, PLXNB1 ELISA Kit, plxnb1a ELISA Kit, Plxnb1 ELISA Kit

Gene ID: 5364

UniProt: O43157