TRADD ELISA Kit

Catalog No. ABIN5690962

Product Details TRADD ELISA Kit

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TRADD ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TRADD.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for TRADD

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Alternative Name: TRADD (TRADD Products)

Synonyms: TRADD ELISA Kit, Hs.89862 ELISA Kit, 9130005N23Rik ELISA Kit, AA930854 ELISA Kit, wu:fc59e02 ELISA Kit, zgc:103556 ELISA Kit, tradd ELISA Kit, TNFRSF1A associated via death domain ELISA Kit, conjugal transfer protein D ELISA Kit, TNFRSF1A-associated via death domain ELISA Kit, TNFRSF1A-associated via death domain L homeolog ELISA Kit, tnfrsf1a-associated via death domain ELISA Kit, TRADD ELISA Kit, traDd ELISA Kit, Tradd ELISA Kit, tradd.L ELISA Kit, tradd ELISA Kit

Gene ID: 8717

UniProt: Q15628

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Hepatitis C