AMPK alpha FLISA Kit

Catalog No. ABIN5691984

Product Details AMPK alpha FLISA Kit

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Binding Specificity: pThr172

Reactivity: Mouse, Human, Rat

Detection Method: Fluorometric

Method Type: Cell ELISA

Application: ELISA

Purpose: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Brand: CytoFluor™

Sample Type: Cell Culture Cells

Analytical Method: Qualitative

Characteristics: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Components: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Application Details

Application Notes: Detection Range: At least 5000 cells/well

Assay Time: 4.5 h

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Expiry Date: 6 months

Target Details

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: AMPKalpha (SNF1A Products)

Synonyms: AMPK FLISA Kit, AMPK2 FLISA Kit, AMPKa2 FLISA Kit, PRKAA FLISA Kit, protein kinase AMP-activated catalytic subunit alpha 2 FLISA Kit, PRKAA2 FLISA Kit

UniProt: Q13131, P54646

Pathways: Warburg Effect