PF4 ELISA Kit

Catalog No. ABIN5692208

Product Details PF4 ELISA Kit

Target: PF4 (Platelet Factor 4 (PF4))

Binding Specificity: AA 30-105

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL4/PF4. 96wells/kit, with removable strips.

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: V30-S105

Sensitivity: < 15 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Pf4 (PF4 Products)

Synonyms: CXCL4 ELISA Kit, PF-4 ELISA Kit, SCYB4 ELISA Kit, Cxcl4 ELISA Kit, Scyb4 ELISA Kit, Pf4a ELISA Kit, RATPF4A ELISA Kit, PF4A ELISA Kit, CXCL4L1 ELISA Kit, CXCL4V1 ELISA Kit, PF4-ALT ELISA Kit, SCYB4V1 ELISA Kit, PF4 ELISA Kit, platelet factor 4 ELISA Kit, platelet factor 4 variant 1 ELISA Kit, PF4 ELISA Kit, Pf4 ELISA Kit, LOC703451 ELISA Kit, PF4V1 ELISA Kit, LOC100524373 ELISA Kit, LOC102181854 ELISA Kit, LOC100630489 ELISA Kit

Background:

Synonyms: Platelet factor 4, PF-4, C-X-C motif chemokine 4, Pf4, Cxcl4, Scyb4

Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Cellular Localisation: Secreted.

UniProt: P06765