PLGF ELISA Kit

Catalog No. ABIN5692237

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse PLGF.

Quick Overview for PLGF ELISA Kit (ABIN5692237)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Binding Specificity: AA 24-158

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details PLGF ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PLGF-2. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21, Immunogen sequence: A24-P158

Sensitivity: < 2 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: Pgf

Background:

Synonyms: Placenta growth factor, PlGF, Pgf, Plgf

Background: Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

Cellular Localisation: Secreted.

UniProt: P49764

Pathways: VEGFR1 Specific Signals