PLGF ELISA Kit

Catalog No. ABIN6719793

Supplier Product No.: koa0292

The Human PLGF ELISA Kit (ABIN6719793) is a Colorimetric ELISA Kit designed to quantify Human PLGF.

Quick Overview for PLGF ELISA Kit (ABIN6719793)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Product Details PLGF ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0292

Supplier: Rockland

Purpose: Human PLGF Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A21-R149

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human PLGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLGF standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.9%, 6%, 5.6%
Inter-Assay Precision (CV%): 9.5%, 6.4%, 5.6%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: PLGF

Background:

Background: The protein, also called PGF, is 149 amino acids long and shares 53 % identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.

Gene Full Name: placental growth factor

Gene ID: 5228

NCBI Accession: NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals