RHOT1 ELISA Kit

Catalog No. ABIN5706906

Product Details RHOT1 ELISA Kit

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.272 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: Mitochondrial Rho GTPase 1 (RHOT1) (RHOT1 Products)

Synonyms: rhot1 ELISA Kit, zgc:55581 ELISA Kit, zgc:77063 ELISA Kit, wu:fd14e11 ELISA Kit, wu:fi14a05 ELISA Kit, arht2 ELISA Kit, miro-2 ELISA Kit, rasl ELISA Kit, ARHT1 ELISA Kit, MIRO-1 ELISA Kit, MIRO1 ELISA Kit, 2210403N23Rik ELISA Kit, AA415293 ELISA Kit, AF244542 ELISA Kit, AI834919 ELISA Kit, Arht1 ELISA Kit, C430039G08Rik ELISA Kit, Miro1 ELISA Kit, miro1 ELISA Kit, si:dkeyp-97g3.6 ELISA Kit, ras homolog family member T1a ELISA Kit, ras homolog family member T2 ELISA Kit, ras homolog family member T1 ELISA Kit, ras homolog family member T1 L homeolog ELISA Kit, rhot1a ELISA Kit, rhot2 ELISA Kit, RHOT1 ELISA Kit, rhot1 ELISA Kit, rhot1.L ELISA Kit, Rhot1 ELISA Kit, rhot1b ELISA Kit

Background: Alternative name: Rac-GTP-binding protein-like protein, Ras homolog gene family member T1, ARHT1, MIRO1, MIRO-1