IL-27 ELISA Kit

Catalog No. ABIN5706908

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-27.

Quick Overview for IL-27 ELISA Kit (ABIN5706908)

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details IL-27 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Analytical Method: Quantitative

Sensitivity: 5.7 pg/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Background: Alternative name: p28, IL30, Interleukin 30, Interleukin-27 subunit alpha