IL-27 ELISA Kit

Catalog No. ABIN6720110

Supplier Product No.: koa0472

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-27.

Quick Overview for IL-27 ELISA Kit (ABIN6720110)

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details IL-27 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0472

Supplier: Rockland

Purpose: Human IL-27 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: EBI3 (R21-K229) + P28 (F29-P243)

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-27 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 5.5%, 5%, 5%
Inter-Assay Precision (CV%): 6.2%, 6.1%, 5.5%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: IL-27

Background:

Background: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T (H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.

Gene Full Name: interleukin 27

Gene ID: 246778

NCBI Accession: NP_663634

UniProt: Q8NEV9