Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Catalog No. ABIN5708882

Quick Overview for Glycogen Colorimetric Assay Kit (Liver/Muscle Samples) (ABIN5708882)

Target: Glycogen

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Liver, Muscle

Analytical Method: Quantitative

Product Details

Detection Range: 1.8 mg/g - 180 mg/g

Minimum Detection Limit: 1.8 mg/g

Purpose: This kit can be used for detection of glycogen content in animal muscle, liver and other samples.

Characteristics: Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.

Material not included: Spectrophotometry (Visible range)

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 7.3%

Intra-assay precision: 3.7%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: 4 °C

Storage Comment: This product can be stored at 2~8°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: Glycogen