Total Bile Acid (TBA) Colorimetric Assay Kit
BCA
Colorimetric
Serum, Tissue Samples
Quantitative
Catalog No. ABIN7121144
$348.00
Plus shipping costs $50.00
Shipping to:
United States
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Quick Overview for Total Bile Acid (TBA) Colorimetric Assay Kit (ABIN7121144)
Target
Total Bile Acide
Detection Method
Colorimetric
Application
Biochemical Assay (BCA)
Sample Type
Serum, Tissue Samples
Analytical Method
Quantitative
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Detection Range
- 1.77 μmol/L - 40 μmol/L
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Minimum Detection Limit
- 1.77 μmol/L
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Purpose
- The Total Bile Acid (TBA) Assay Kit is biochemical kit in Colorimetric method,can be used for in vitro quantitative determination of total bile acids content in serum.
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Sensitivity
- 1.36 μmol/L
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Characteristics
- With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
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Material not included
- Spectrophotometry (Visible range), Biochemistry analyzer
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Assay Time
- 25 min
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Assay Precision
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Inter-assay precision: 3.7%
Intra-assay precision: 3.4%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- 4 °C
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Storage Comment
- This product can be stored at 2~8°C for 12 months with shading light.
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Expiry Date
- 12 months
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- Total Bile Acide
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Alternative Name
- Total Bile Acid
Target
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