BCA Protein Colorimetric Assay Kit

Catalog No. ABIN5708967

Quick Overview for BCA Protein Colorimetric Assay Kit (ABIN5708967)

Target: Bicinchoninic Acid

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.0165 mg/mL - 1 mg/mL

Minimum Detection Limit: 0.0165 mg/mL

Purpose: The Total Protein Assay Kit (With standard, BCA method) is biochemical kit in Colorimetric method

Characteristics: Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Material not included: Microplate reader

Application Details

Assay Time: < 1 h

Assay Precision:

Inter-assay precision: 4.5%

Intra-assay precision: 2.2%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: RT

Storage Comment: This product can be stored at room temperature for 12 months with shading light.

Expiry Date: 12 months

References

Uysal, Yuksel, Kizildag, Yuce, Gumus, Karakilic, Guvendi, Koc, Kandis, Ates: "Regular aerobic exercise correlates with reduced anxiety and incresed levels of irisin in brain and white adipose tissue." in: Neuroscience letters, Vol. 676, pp. 92-97, (2019) (PubMed).

Target Details

Target: Bicinchoninic Acid

Alternative Name: BCA