TSH ELISA Kit

Catalog No. ABIN577072

Human TSH ELISA Kit, Colorimetric assay for quantification of Human TSH.

Quick Overview for TSH ELISA Kit (ABIN577072)

Target: TSH (Thyroid Stimulating Hormone (TSH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details TSH ELISA Kit

Purpose: This TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to

Analytical Method: Quantitative

Sensitivity: The minimal detectable concentration of TSH by this assay is estimated to be 0.2 IU/mL.

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for TSH

Target: TSH (Thyroid Stimulating Hormone (TSH))

Alternative Name: Thyroid Stimulating Hormone (TSH) regular

Target Type: Hormone

Background: Thyroxine (T4) and Triiodothyronine (T3) are secreted from the thyroid gland and regulated by a sensitive feedback system involving the hypothalamus and pituitary gland. The hypothalamus releases the thyrotropin releasing hormone (TRH), which stimulates the pituitary to release the thyroid stimulating hormone (TSH). This causes the thyroid to release T3 and T4 and these in turn regulate the release of TRH and TSH via a feedback control mechanism. When required, they are released into the bloodstream where they bind largely to specific binding proteins, which act as a mechanism of transport. The two major proteins are Thyroxine Binding Globulin (TBG) and Thyroxine Binding Pre-Albumin (TBPA). A small percentage of this circulating T4 and T3 remains unbound or free and this is the portion of the hormones believed to be physiologically active. Any abnormalities in the thyroid, pituitary or hypothalamus glands may alter the levels of these hormones in the bloodstream