CEA ELISA Kit

Catalog No. ABIN577107

The Human CEA ELISA Kit (ABIN577107) is a Colorimetric ELISA Kit designed to quantify Human CEA.

Quick Overview for CEA ELISA Kit (ABIN577107)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details CEA ELISA Kit

Purpose: This CEA enzyme linked immunosorbent assay (ELISA) kit uses a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for CEA. During the assay, standards or samples are added to the microtiter plate wells and CEA, if present, will bind to the CEA antibody pre-coated on the wells. Then, a preparation of horseradish peroxidase (HRP)-conjugated CEA antibody is added to each well. The conjugated antibody will bind to the CEA immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Afterwards, 3, 3', 5, 5' tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the CEA content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change s i measured spectrophotometrically at the wavelength of 450nm 2nm. In order to quantify the amount of CEA present in the sample, the calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus CEA concentration (ng/mL). The concentration of CEA in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(01) 2

Analytical Method: Quantitative

Sensitivity: The minimal detectable dose of this CEA assay was calculated by adding two standard deviations to the mean optical density value of 20 zero standard replicates and determining the corresponding concentration from the standard curve. The minimal detectable dose for this human CEA assay generated by this method is 0.724ng/ml.

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: Carcinoembryonic Antigen (CEA)

Background: Interleukin 13 (IL-13) is produced primarily by activated T lymphocytes. It shows approximately 3% amino acid (aa) sequence identity with IL-4 and shares many biological properties with IL-4. IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. It can suppress the cytotoxic functions of monocytes/macrophages and the production of proinflammatory cytokines by monocytes/macrophages. Although human or mouse IL-13 has effects similar to those of IL-4 on human B cells, mouse IL-13 has no effect on mouse B cells. Unlike IL-4, which is a growth and differentiation factor for human and mouse T cells, IL-13 has no effects on either human or mouse T cells. This IL-13 ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IL-13 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 5 pg/mL. It showed no cross reactivity with other cytokines tested. This IL-13 ELISA is expected to be effectively used for further investigations into the relationship between IL-15 and the various conditions mentioned