alpha Fetoprotein ELISA Kit
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- Target See all alpha Fetoprotein (AFP) ELISA Kits
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- This AFP enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AFP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. AFP if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound AFP and other components of sample. In order to quantitate the amount of AFP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for AFP is added to each well to
- Analytical Method
- Quantitative
- Sensitivity
- The minimum detectable dose of AFP was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human AFP using a standard curve generated is 2.0 ng/mL .
- Components
- Standards: 1 set/2 vials
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- Discover our best selling AFP ELISA Kit
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- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Preservative
- Without preservative
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- Target See all alpha Fetoprotein (AFP) ELISA Kits
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Alternative Name
- Alpha Fetoprotein (AFP) (AFP Products)
- Synonyms
- FETA ELISA Kit, HPAFP ELISA Kit, alpha fetoprotein ELISA Kit, alpha-fetoprotein ELISA Kit, AFP ELISA Kit, Afp ELISA Kit
- Pathways
- C21-Steroid Hormone Metabolic Process
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