alpha Fetoprotein ELISA Kit

Catalog No. ABIN577112

This Colorimetric ELISA kit is designed for the quantitative measurement of Human alpha Fetoprotein.

Quick Overview for alpha Fetoprotein ELISA Kit (ABIN577112)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details alpha Fetoprotein ELISA Kit

Purpose: This AFP enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AFP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. AFP if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound AFP and other components of sample. In order to quantitate the amount of AFP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for AFP is added to each well to

Analytical Method: Quantitative

Sensitivity: The minimum detectable dose of AFP was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human AFP using a standard curve generated is 2.0 ng/mL .

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Alpha Fetoprotein (AFP)

Pathways: C21-Steroid Hormone Metabolic Process