Mix-n-Stain™ CF®680 Small Ligand Labeling Kit
Quick Overview for Mix-n-Stain™ CF®680 Small Ligand Labeling Kit (ABIN6199171)
Application
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Conjugate
- CF®680
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Purpose
- Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.
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Brand
- Mix-n-Stain™
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Specificity
- Mix-n-Stain small ligand labeling kits are optimized for labeling small ligands with an amino group.
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Characteristics
- Mix-n-Stain™ CF® dye small ligand labeling kits are designed for rapid, covalent labeling of low molecular weight (Mwt ~ 150 - 5,000) and relatively high affinity biological ligands (or substrates) without a final purification step. Simply mix your ligand with the CF® dye of your choice and after a 30 minute incubation and a brief quenching step (no purification required), you will have a covalently labeled dye-ligand conjugate for protein labeling that performs as well as synthetic fluorescent ligands from leading suppliers, at a fraction of the cost. Even without column purification, the CF® dye-ligand does not non-specifically stain cells or other biomolecules. Suitable ligands or substrates include the SNAP-tag®, CLIPtag™, HaloTag® and TMP-tag ligands that have an aliphatic amine. Many other small ligands are also possible candidates for Mix-n-Stain™ labeling, if they meet the criteria described in the product protocol.
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Components
- Reactive CF dye, Reaction Buffer, Quenching Buffer, DMSO (anhydrous)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Storage
- -20 °C
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Storage Comment
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Storage: -20 °C
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Expiry Date
- 6 months
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