Adrenomedullin ELISA Kit

Catalog No. ABIN6244353

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Rat and Mouse Adrenomedullin.

Quick Overview for Adrenomedullin ELISA Kit (ABIN6244353)

Target: Adrenomedullin (ADM)

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details Adrenomedullin ELISA Kit

Purpose: Human/Mouse/Rat Adrenomedullin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat Adrenomedullin

Sensitivity: 0.9 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for Adrenomedullin

Target: Adrenomedullin (ADM)

Alternative Name: Adrenomedullin

Target Type: Hormone

Background:

Gene Symbols: ADM, AM

Background: Adrenomedullin (ADM or AM) consists of 52 amino acids, and is a potent hypotensive and vasodilatator agent. It was initially isolated in 1993 from a pheochromocytoma, a tumor of the adrenal medulla. ADM is found in blood in a considerable concentration and may function as a hormone related to the physiologic control of fluid and electrolyte homeostasis. It has also been reported that ADM stimulates the growth of new blood vessels (angiogenesis) and increasing the tolerance of cells to oxidative stress and hypoxic injury.

Protein Name (Synonyms): ADM [Cleaved into: Adrenomedullin (A

Gene ID: 133

Pathways: Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation