CCL1 ELISA Kit

Catalog No. ABIN624994

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CCL1. There are 3 publications available.

Quick Overview for CCL1 ELISA Kit (ABIN624994)

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 4-3000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details CCL1 ELISA Kit

Minimum Detection Limit: 4 pg/mL

Purpose: Human I-309 (TCA-3/CCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 4 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for CCL1 ELISA Kit (ABIN624994)

Das, Sarrou, Podgrabinska, Cassella, Mungamuri, Feirt, Gordon, Nagi, Wang, Entenberg, Condeelis, Skobe: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." in: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).

Leow, Goh, Heng: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).

Celebi, Mantovani, Pineault: "Irradiated Mesenchymal Stem Cells improve the ex vivo expansion of Hematopoietic Progenitors by partly mimicking the bone marrow endosteal environment." in: Journal of immunological methods, Vol. 370, Issue 1-2, pp. 93-103, (2011) (PubMed).

Target Details for CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Alternative Name: I-309

Background: Gene Names: CCL1 SCYA1
Protein names: C-C motif chemokine 1 (Small-inducible cytokine A1) (T lymphocyte-secreted protein I-309)

Gene ID: 6346

UniProt: P22362