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MMP1 ELISA Kit
MMP1
Reactivity: Human
Colorimetric
Sandwich ELISA
8-18000 pg/mL
Cell Lysate, Tissue Lysate
Product Details MMP1 ELISA Kit
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Target
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MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
Reactivity
All reactivities for MMP1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
8-18000 pg/mL
Minimum Detection Limit
8 pg/mL
Application
ELISA
Purpose
Human MMP-1 ELISA Kit for cell and tissue lysate samples.
Sample Type
Tissue Lysate, Cell Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes human MMP-1.
Sensitivity
8 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for MMP1
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Target
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MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
Alternative Name
MMP-1 (MMP1 Products )
Synonyms
CLG ELISA Kit, CLGN ELISA Kit, Mmp1a ELISA Kit, CG4859 ELISA Kit, Dm1-MMP ELISA Kit, Dmel\\CG4859 ELISA Kit, MMP-1 ELISA Kit, MMP1 ELISA Kit, Mmp 1 ELISA Kit, dMMP1 ELISA Kit, dm1-MMP ELISA Kit, dmmp1 ELISA Kit, l(2)k04809 ELISA Kit, mmp1 ELISA Kit, Mmp1 ELISA Kit, Mcol-A ELISA Kit, Mcola ELISA Kit, col4 ELISA Kit, mmp18 ELISA Kit, Clgn ELISA Kit, matrix metallopeptidase 1 ELISA Kit, Matrix metalloproteinase 1 ELISA Kit, matrix metalloproteinase 1 ELISA Kit, matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1a (interstitial collagenase) ELISA Kit, matrix metallopeptidase 1 S homeolog ELISA Kit, interstitial collagenase ELISA Kit, matrix metallopeptidase 8 L homeolog ELISA Kit, MMP1 ELISA Kit, Mmp1 ELISA Kit, RB11133 ELISA Kit, Mmp1a ELISA Kit, mmp1.S ELISA Kit, LOC100727966 ELISA Kit, mmp8.L ELISA Kit
Background
Gene Names: MMP1 CLG Protein names: Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]
Gene ID
4312
UniProt
P03956
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