Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TIMP1 ELISA Kit

TIMP1 Reactivity: Human Colorimetric Sandwich ELISA 30-18.000 pg/mL Cell Lysate, Tissue Lysate
Catalog No. ABIN625099
  • Target See all TIMP1 ELISA Kits
    TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
    Reactivity
    • 9
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    30-18.000 pg/mL
    Minimum Detection Limit
    30 pg/mL
    Application
    ELISA
    Purpose
    Human TIMP-1 ELISA Kit for cell and tissue lysate samples.
    Sample Type
    Tissue Lysate, Cell Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human TIMP-1.
    Sensitivity
    30 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
    Featured
    Discover our best selling TIMP1 ELISA Kit
    Top Product
    Discover our top product TIMP1 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all TIMP1 ELISA Kits
    TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
    Alternative Name
    TIMP-1 (TIMP1 Products)
    Synonyms
    TIMP-1 ELISA Kit, TIMP1 ELISA Kit, DKFZp468A0912 ELISA Kit, CLGI ELISA Kit, EPA ELISA Kit, EPO ELISA Kit, HCI ELISA Kit, TIMP ELISA Kit, Timp ELISA Kit, Clgi ELISA Kit, TIMP metallopeptidase inhibitor 1 ELISA Kit, tissue inhibitor of metalloproteinase 1 ELISA Kit, TIMP1 ELISA Kit, Timp1 ELISA Kit
    Background
    Gene Names: TIMP1 CLGI TIMP
    Protein names: Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
    Gene ID
    7076
    UniProt
    P01033
You are here:
Support