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Nociceptin ELISA Kit

PNOC Reactivity: Human, Rat, Mouse Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
Catalog No. ABIN6386187
  • Target See all Nociceptin (PNOC) ELISA Kits
    Nociceptin (PNOC)
    Reactivity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    0.1 ng/mL - 1000 ng/mL
    Minimum Detection Limit
    0.1 ng/mL
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Nociceptin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This EIA kit is designed to detect human, mouse, and rat Nociceptin
    Sensitivity
    0.4 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Expiry Date
    6 months
  • Target See all Nociceptin (PNOC) ELISA Kits
    Nociceptin (PNOC)
    Alternative Name
    Nociceptin (PNOC Products)
    Synonyms
    PPNOC ELISA Kit, N/OFQ ELISA Kit, N23K ELISA Kit, Npnc1 ELISA Kit, OFQ/N ELISA Kit, pnoc ELISA Kit, zgc:136385 ELISA Kit, OFQ ELISA Kit, prepronociceptin ELISA Kit, prepronociceptin b ELISA Kit, PNOC ELISA Kit, Pnoc ELISA Kit, pnocb ELISA Kit
    Gene ID
    5368
    UniProt
    Q13519
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