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DKK1 ELISA Kit

Rockland koa0561 DKK1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Catalog No. ABIN6719676
Supplier Product No.: koa0561
  • Target See all DKK1 ELISA Kits
    DKK1 (Dickkopf Homolog 1 (DKK1))
    Reactivity
    • 11
    • 11
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    46.9 pg/mL - 3000 pg/mL
    Minimum Detection Limit
    46.9 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0561
    Supplier
    Rockland
    Purpose
    Mouse DKK1 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant mouse DKK1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 15 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product DKK1 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each mouse DKK1 standard solution and each sample is measured in duplicate.
    Comment

    Standard: Expression system for standard: CHO, Immunogen sequence: S30-H272

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all DKK1 ELISA Kits
    DKK1 (Dickkopf Homolog 1 (DKK1))
    Alternative Name
    DKK1 (DKK1 Products)
    Background

    Synonyms: Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK

    Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

    Gene ID
    13380
    NCBI Accession
    NP_034181
    UniProt
    O54908
    Pathways
    WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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