TIMP4 ELISA Kit

Catalog No. ABIN6719680

Product Details TIMP4 ELISA Kit

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Metalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4

Background: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What's more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Gene Name: TIMP4

Production: Natural and recombinant mouse TIMP-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: C30-P224

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5,000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL mouse TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse TIMP-4 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Alternative Name: TIMP-4 (TIMP4 Products)

Synonyms: TIMP4 ELISA Kit, TIMP-4 ELISA Kit, timp4 ELISA Kit, TIMP metallopeptidase inhibitor 4 ELISA Kit, TIMP metallopeptidase inhibitor 4, tandem duplicate 3 ELISA Kit, tissue inhibitor of metalloproteinase 4 ELISA Kit, TIMP4 ELISA Kit, timp4.3 ELISA Kit, Timp4 ELISA Kit

Gene ID: 110595

NCBI Accession: NP_542370

UniProt: Q9JHB3