FLT3LG ELISA Kit

Catalog No. ABIN6719683

Supplier Product No.: koa0173

Mouse FLT3LG ELISA Kit Colorimetric assay for quantification of Mouse FLT3LG.

Quick Overview for FLT3LG ELISA Kit (ABIN6719683)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details FLT3LG ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0173

Supplier: Rockland

Purpose: Mouse Flt-3 ligand Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse Flt-3 ligand. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse Flt-3 ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse Flt-3 ligand standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: G27-R188

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for FLT3LG

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt-3 ligand

Background:

Synonyms: Flt 3 ligand, Flt 3L, Flt3 L, FLT3 LG, Flt3 ligand, Flt3L, FLT3L_HUMAN, Flt3lg, Fms related tyrosine kinase 3 ligand, Fms-related tyrosine kinase 3 ligand, SL cytokine

Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3 ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3 ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3 ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3 ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Gene ID: 14256

NCBI Accession: NP_038548

UniProt: P49772

Pathways: RTK Signaling