F11R ELISA Kit

Catalog No. ABIN6719688

Supplier Product No.: koa0813

The Human F11R ELISA Kit (ABIN6719688) is a Colorimetric ELISA Kit designed to quantify Human F11R. This ELISA Kit has been cited in 1 publication.

Quick Overview for F11R ELISA Kit (ABIN6719688)

Target: F11R (F11 Receptor (F11R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details F11R ELISA Kit

Minimum Detection Limit: 46.9 pg/mL

Supplier Product No.: koa0813

Supplier: Rockland

Purpose: Human JAM-A Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S28-V238

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. We recommend that each human JAM-A standard solution and each sample is measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.2%, 5.2%, 5.3%
Inter-Assay Precision (CV%): 7.3%, 5.3%, 7.7%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for F11R ELISA Kit (ABIN6719688)

Bluhmki, Bitzer, Gindele, Schruf, Kiechle, Webster, Schymeinsky, Ries, Gantner, Bischoff, Garnett, Heilker: "Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model." in: Scientific reports, Vol. 10, Issue 1, pp. 13022, (2020) (PubMed).

Target Details for F11R

Target: F11R (F11 Receptor (F11R))

Alternative Name: JAM-A

Background:

Background: Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Gene Full Name: F11 receptor

Gene ID: 50848

NCBI Accession: NP_058642

UniProt: Q9Y624

Pathways: Cell-Cell Junction Organization