Kallikrein 8 ELISA Kit

Catalog No. ABIN6719701

Product Details Kallikrein 8 ELISA Kit

Target: Kallikrein 8 (KLK8)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: Brain serine protease 1, BSP1, hK8, HNP, HPN, Kallikrein 8 (neuropsin/ovasin), Kallikrein related peptidase 8, KLK8 protein type 1, KLK8 protein type 2, KLK8_HUMAN, Neuropsin, Neuropsin type 1, Neuropsin type 2, NP, NRPN, Ovasin, PRO322, Protease serine 19, PRSS 19, Serine protease 19, Serine protease kallikrein, Serine protease kallikrein/ovasin/neuropsin, Serine protease TADG 14, TADG14, Tumor associated differentially expressed gene 14, Tumor associated differentially expressed gene 14 protein

Background: Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

Gene Name: KLK8

Production: Natural and recombinant human KLK8. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q29-G260

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK8 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Kallikrein 8

Target: Kallikrein 8 (KLK8)

Alternative Name: KLK8 - Kallikrein-8 (KLK8 Products)

Synonyms: HNP ELISA Kit, NP ELISA Kit, NRPN ELISA Kit, PRSS19 ELISA Kit, TADG14 ELISA Kit, bsp1 ELISA Kit, KLK8 ELISA Kit, BSP1 ELISA Kit, Nrpn ELISA Kit, Prss19 ELISA Kit, kallikrein related peptidase 8 ELISA Kit, kallikrein related-peptidase 8 ELISA Kit, KLK8 ELISA Kit, Klk8 ELISA Kit

Gene ID: 11202

NCBI Accession: NP_001268360

UniProt: O60259

Pathways: Complement System