BMP7 ELISA Kit

Catalog No. ABIN6719712

Product Details BMP7 ELISA Kit

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: BMP 7, BMP7, BMP-7, Bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, OP1-, Osteogenic protein 2

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Gene Name: BMP-7

Production: Natural and recombinant rat BMP-7. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: S292-H430

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each rat BMP-7 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP-7 (BMP7 Products)

Synonyms: bmp-7 ELISA Kit, bmp7 ELISA Kit, op-1 ELISA Kit, BMP7 ELISA Kit, zgc:153025 ELISA Kit, OP-1 ELISA Kit, OP1 ELISA Kit, BMP-7 ELISA Kit, fb83f09 ELISA Kit, wu:fb83f09 ELISA Kit, bone morphogenetic protein 7 ELISA Kit, bone morphogenetic protein 7, gene 1 L homeolog ELISA Kit, bone morphogenetic protein 7b ELISA Kit, bone morphogenetic protein 7, gene 2 L homeolog ELISA Kit, bone morphogenetic protein 7a ELISA Kit, BMP7 ELISA Kit, bmp7.1.L ELISA Kit, LOC100136282 ELISA Kit, bmp7b ELISA Kit, Bmp7 ELISA Kit, LOC100008826 ELISA Kit, bmp7.2.L ELISA Kit, bmp7a ELISA Kit

Gene ID: 85272

UniProt: G3V6W8

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance