TIMP1 ELISA Kit

Catalog No. ABIN6719721

Supplier Product No.: koa0357

This Rat TIMP1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat TIMP1.

Quick Overview for TIMP1 ELISA Kit (ABIN6719721)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details TIMP1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0357

Supplier: Rockland

Purpose: Rat TIMP-1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant rat TIMP-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 3 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TIMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat TIMP-1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: C24-A217

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP-1

Background:

Synonyms: C-C chemokine receptor type 5, CC-CKR-5, CCR5, CCR5_HUMAN, CHEMR13, CLGI, Collagenase inhibitor, EPA, EPO, Erythroid potentiating activity, Fibroblast collagenase inhibitor, HCI, HIV-1 fusion coreceptor, Human Collagenase Inhibitor, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, TIMP, TIMP metallopeptidase inhibitor 1, TIMP1 protein, Tissue inhibitor of metalloproteinases, Tissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor

Background: The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.

Gene ID: 116510

NCBI Accession: NP_446271

UniProt: P30120