TGFB2 ELISA Kit

Catalog No. ABIN6719755

Supplier Product No.: koa0609

Human TGFB2 ELISA Kit Colorimetric assay for quantification of Human TGFB2.

Quick Overview for TGFB2 ELISA Kit (ABIN6719755)

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Product Details TGFB2 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0609

Supplier: Rockland

Purpose: Human TGF beta 2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human TGF-beta 2. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TGF-beta 2 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A303-S414

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TGFB2

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Alternative Name: TGF beta 2

Background:

Synonyms: BSC 1 cell growth inhibitor, BSC-1 cell growth inhibitor, Cetermin, G-TSF, Glioblastoma derived T cell suppressor factor, Glioblastoma-derived T-cell suppressor factor, Polyergin, TGF beta2, TGF-beta-2, TGFB2, TGFB2_HUMAN, Transforming growth factor beta-2, Transforming growth factor, beta 2

Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Gene ID: 7042

NCBI Accession: NP_001129071

UniProt: P61812

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus