NRG1-beta 1 ELISA Kit

Catalog No. ABIN6719765

Supplier Product No.: koa0835

This Colorimetric ELISA kit is designed for the quantitative measurement of Human NRG1-beta 1.

Quick Overview for NRG1-beta 1 ELISA Kit (ABIN6719765)

Target: NRG1-beta 1 (Neuregulin-1 beta 1 (NRG1-beta 1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details NRG1-beta 1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0835

Supplier: Rockland

Purpose: Human Neuregulin-1 - NRG1-beta1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S2-K246

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Neuregulin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Neuregulin-1 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.7%, 4.9%, 4.3%
Inter-Assay Precision (CV%): 9.1%, 6.6%, 5%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for NRG1-beta 1

Target: NRG1-beta 1 (Neuregulin-1 beta 1 (NRG1-beta 1))

Alternative Name: Neuregulin-1/NRG1-beta1

Background:

Background: Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.

Gene Full Name: neuregulin 1

Gene ID: 3084

NCBI Accession: NP_001153467

UniProt: Q02297