EpCAM ELISA Kit

Catalog No. ABIN6719777

Product Details EpCAM ELISA Kit

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7 pg/mL - 6000 pg/mL

Minimum Detection Limit: 93.7 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 17 1A, 323/A3, AUA1, CD326, CD326 antigen, CO 17A, CO17 1A, CO17A, DIAR5, EGP2, EGP, EGP2, EGP314, EGP40, Ep CAM, Ep-CAM, EPCAM, ESA, GA733 1, GA733 2, GA733-2, hEGP 2, hEGP314, HNPCC8, KS1/4, KSA, M1S 1, M1S2 ,M4S1, MIC18 MK 1 ,TACD1, TACSTD1, TROP1

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Gene Name: EPCAM

Production: Natural and recombinant human EPCAM. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q24-K265

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human EPCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EPCAM standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 93.7pg/mL-6000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EPCAM (EPCAM Products)

Synonyms: DIAR5 ELISA Kit, EGP-2 ELISA Kit, EGP314 ELISA Kit, EGP40 ELISA Kit, ESA ELISA Kit, HNPCC8 ELISA Kit, KS1/4 ELISA Kit, KSA ELISA Kit, M4S1 ELISA Kit, MIC18 ELISA Kit, MK-1 ELISA Kit, TACSTD1 ELISA Kit, TROP1 ELISA Kit, ECS-1 ELISA Kit, ECS1 ELISA Kit, ESA1 ELISA Kit, M17S1 ELISA Kit, EGP ELISA Kit, Ep-CAM ELISA Kit, sb:cb6 ELISA Kit, tacstd ELISA Kit, wu:fj17g02 ELISA Kit, zgc:110304 ELISA Kit, zgc:77119 ELISA Kit, tacstd1 ELISA Kit, MGC80540 ELISA Kit, EPCAM ELISA Kit, egp ELISA Kit, ksa ELISA Kit, m4s1 ELISA Kit, mk-1 ELISA Kit, cd326 ELISA Kit, egp40 ELISA Kit, mic18 ELISA Kit, trop1 ELISA Kit, ep-cam ELISA Kit, hegp-2 ELISA Kit, co17-1a ELISA Kit, ga733-2 ELISA Kit, CD326 ELISA Kit, Egp314 ELISA Kit, EpCAM1 ELISA Kit, GA733-2 ELISA Kit, Ly74 ELISA Kit, Tacsd1 ELISA Kit, Tacstd1 ELISA Kit, gp40 ELISA Kit, epithelial cell adhesion molecule ELISA Kit, flotillin 2 ELISA Kit, epithelial cell adhesion molecule S homeolog ELISA Kit, EPCAM ELISA Kit, FLOT2 ELISA Kit, epcam ELISA Kit, epcam.S ELISA Kit, Epcam ELISA Kit

Gene ID: 4072

NCBI Accession: NP_002345

UniProt: P16422