Tissue factor ELISA Kit

Catalog No. ABIN6719791

Product Details Tissue factor ELISA Kit

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: CD142, Coagulation factor III, F3, TF, TF_HUMAN, TFA, Thromboplastin, Tissue factor

Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Gene Name: F3

Production: Natural and recombinant human Tissue factor. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S33-E251

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Tissue factor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) cell lysates or urine to each empty well. It is recommended that each human Tissue factor standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Tissue factor

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: Tissue factor - F3 (F3 Products)

Synonyms: CD142 ELISA Kit, TF ELISA Kit, TFA ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, f3 ELISA Kit, AA409063 ELISA Kit, Cf-3 ELISA Kit, Cf3 ELISA Kit, tf ELISA Kit, zgc:112151 ELISA Kit, coagulation factor III, tissue factor ELISA Kit, transferrin ELISA Kit, coagulation factor IIIa ELISA Kit, coagulation factor III ELISA Kit, tissue factor ELISA Kit, coagulation factor IIIb ELISA Kit, coagulation factor III (thromboplastin, tissue factor) S homeolog ELISA Kit, F3 ELISA Kit, TF ELISA Kit, f3a ELISA Kit, tf ELISA Kit, f3b ELISA Kit, f3.S ELISA Kit

Gene ID: 2152

NCBI Accession: NP_001171567

UniProt: P13726

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling