ICAM2 ELISA Kit

Catalog No. ABIN6719803

Product Details ICAM2 ELISA Kit

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: Intercellular adhesion molecule 2, Ly60, CD102

Background: Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Gene Name: ICAM2

Production: Natural and recombinant human ICAM-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: K25-Q223

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ICAM-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human ICAM-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for ICAM2

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Alternative Name: ICAM-2 (ICAM2 Products)

Synonyms: ICAM2 ELISA Kit, CD102 ELISA Kit, Icam-2 ELISA Kit, Ly-60 ELISA Kit, intercellular adhesion molecule 2 ELISA Kit, LOC506088 ELISA Kit, ICAM2 ELISA Kit, LOC616254 ELISA Kit, LOC100140873 ELISA Kit, Icam2 ELISA Kit

Gene ID: 3384

NCBI Accession: NP_000864

UniProt: P13598