EPO ELISA Kit

Catalog No. ABIN6719829

Supplier Product No.: koa0815

This Rat EPO ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat EPO.

Quick Overview for EPO ELISA Kit (ABIN6719829)

Target: EPO (Erythropoietin (EPO))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate

Product Details EPO ELISA Kit

Minimum Detection Limit: 46.9 pg/mL

Supplier Product No.: koa0815

Supplier: Rockland

Purpose: Rat EPO Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant rat EPO. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin) or tissue homogenates to each empty well. We recommend that each rat EPO standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: T44-K226

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background:

Synonyms: EP, EPO, EPO_HUMAN, Epoetin, Erythropoietin, MVCD2

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Gene ID: 24335

NCBI Accession: NP_058697

UniProt: P29676

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity