EPO ELISA Kit

Catalog No. ABIN6719830

Supplier Product No.: koa0162

Mouse EPO ELISA Kit Colorimetric assay for quantification of Mouse EPO.

Quick Overview for EPO ELISA Kit (ABIN6719830)

Target: EPO (Erythropoietin (EPO))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Lysates

Product Details EPO ELISA Kit

Minimum Detection Limit: 46.9 pg/mL

Supplier Product No.: koa0162

Supplier: Rockland

Purpose: Mouse EPO Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse EPO. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 46.9pg/mL-3000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A27-R192

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background:

Synonyms: C C motif chemokine 24, CCL24, Chemokine (C C motif) ligand 24, Chemokine CC Motif Ligand 2, CK beta 6, Ckb 6, Ckb6, Eosinophil chemotactic protein 2, Eotaxin 2 like protein, MPIF 2, MPIF2, Myeloid progenitor inhibitory factor 2, OTTHUMP00000210225, SCYA24, Small inducible cytokine A24, Small inducible cytokine subfamily A (Cys Cys) member 24

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Gene ID: 13856

NCBI Accession: NP_031968

UniProt: P07321

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity