CD47 ELISA Kit

Catalog No. ABIN6719833

Product Details CD47 ELISA Kit

Target: CD47

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Antigen identified by monoclonal antibody 1D8, Antigenic surface determinant protein OA3, CD 47, CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 Molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen

Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Gene Name: CD47

Production: Natural and recombinant human CD47. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-P139

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD47 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human CD47 standard solution and each sample be measured in duplicate.

ELISA Dilution: 125pg/mL-8000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD47

Target: CD47

Alternative Name: CD47 (CD47 Products)

Synonyms: IAP ELISA Kit, MER6 ELISA Kit, OA3 ELISA Kit, 9130415E20Rik ELISA Kit, AA407862 ELISA Kit, AI848868 ELISA Kit, AW108519 ELISA Kit, B430305P08Rik ELISA Kit, Itgp ELISA Kit, CD47/IAP ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, CD47 molecule ELISA Kit, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) ELISA Kit, Cd47 molecule ELISA Kit, ALPI ELISA Kit, CD47 ELISA Kit, Cd47 ELISA Kit

Gene ID: 961

NCBI Accession: NP_001768

UniProt: Q08722