Neurotrophin 4 ELISA Kit

Catalog No. ABIN6719849

Supplier Product No.: koa0282

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Neurotrophin 4.

Quick Overview for Neurotrophin 4 ELISA Kit (ABIN6719849)

Target: Neurotrophin 4 (NTF4)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Neurotrophin 4 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0282

Supplier: Rockland

Purpose: Human NT-4 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human NT-4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: G81-A210

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NT-4

Background:

Synonyms: GLC10, GLC1O, Neurotrophic factor 4, Neurotrophic factor 5, Neurotrophin 5, Neurotrophin 5 (neurotrophin 4/5), Neurotrophin-4, Neurotrophin-5, Neutrophic factor 4, Neutrophic factor 5, NT 4, NT 4/5, NT 5, NT-4, NT-5,NT4/5, NT4P, NT5, NTF4, NTF4_HUMAN, NTF5

Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Gene ID: 4909

NCBI Accession: NP_006170

UniProt: P34130

Pathways: RTK Signaling