BMP2 ELISA Kit

Catalog No. ABIN6719858

Supplier Product No.: koa0151

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat BMP2.

Quick Overview for BMP2 ELISA Kit (ABIN6719858)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Osseous Tissue, Serum

Product Details BMP2 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0151

Supplier: Rockland

Purpose: Rat BMP-2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates or serum to each empty well. It is recommended that each rat BMP-2 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7%, 4.6%, 6.1%
Inter-Assay Precision (CV%): 8.8%, 6.4%, 6.8%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP-2

Background:

Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Gene Full Name: bone morphogenetic protein 2

Gene ID: 29373

NCBI Accession: NP_058874

UniProt: P49001

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation