TLR3 ELISA Kit

Catalog No. ABIN6719884

Supplier Product No.: koa0547

Human TLR3 ELISA Kit Colorimetric assay for quantification of Human TLR3.

Quick Overview for TLR3 ELISA Kit (ABIN6719884)

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Product Details TLR3 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0547

Supplier: Rockland

Purpose: Human TLR3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human TLR3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR3 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: K27-S711

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TLR3

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name: TLR3

Background:

Synonyms: CD_antigen=CD283, CD283, IIAE2, Toll like receptor 3

Background: Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.

Gene ID: 7098

NCBI Accession: NP_003256

UniProt: O15455

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades