Galectin 2 ELISA Kit

Catalog No. ABIN6719894

Product Details Galectin 2 ELISA Kit

Target: Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: Beta galactoside binding lectin L14 II, Beta-galactoside-binding lectin L-14-II, CBP35, Gal-2, GAL3, GALBP, Galectin 2, Galectin-2, GALIG, HL14, Lactose binding lectin 2, Lactose-binding lectin 2, LEG2_HUMAN, Lgals2, MAC2, S Lac lectin 2, S-Lac lectin 2

Background: Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Gene Name: LGALS2

Production: Natural and recombinant human Galectin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E132

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Galectin 2

Target: Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

Alternative Name: Galectin-2 (LGALS2 Products)

Synonyms: LGALS3 ELISA Kit, galectin-2 ELISA Kit, HL14 ELISA Kit, 2200008F12Rik ELISA Kit, AI324147 ELISA Kit, LGALS2 ELISA Kit, hl14 ELISA Kit, Drgal1-L3 ELISA Kit, lgals1l3 ELISA Kit, zgc:66283 ELISA Kit, Drgal1-L2 ELISA Kit, lgals1l2 ELISA Kit, zgc:86752 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose-binding, soluble 2 ELISA Kit, lectin, galactoside binding soluble 2 ELISA Kit, lectin, galactoside-binding, soluble, 2b ELISA Kit, lectin, galactoside-binding, soluble, 2a ELISA Kit, LGALS2 ELISA Kit, Lgals2 ELISA Kit, lgals2 ELISA Kit, lgals2b ELISA Kit, lgals2a ELISA Kit

Gene ID: 3957

NCBI Accession: NP_006489

UniProt: P05162