Fetuin A ELISA Kit

Catalog No. ABIN6719928

Supplier Product No.: koa0448

The Human Fetuin A ELISA Kit (ABIN6719928) is a Colorimetric ELISA Kit designed to quantify Human Fetuin A.

Quick Overview for Fetuin A ELISA Kit (ABIN6719928)

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details Fetuin A ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Supplier Product No.: koa0448

Supplier: Rockland

Purpose: Human Fetuin A Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: A19-V367

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Fetuin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human Fetuin A standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.7%, 4%, 4%
Inter-Assay Precision (CV%): 8.4%, 4.5%, 5.8%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Fetuin A

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Alternative Name: Fetuin A

Background:

Background: Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Gene Full Name: alpha 2-HS glycoprotein

Gene ID: 197

NCBI Accession: NP_001613

UniProt: P02765