IL-7 ELISA Kit

Catalog No. ABIN6719941

Supplier Product No.: koa0460

Mouse IL-7 ELISA Kit Colorimetric assay for quantification of Mouse IL-7.

Quick Overview for IL-7 ELISA Kit (ABIN6719941)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Serum

Product Details IL-7 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0460

Supplier: Rockland

Purpose: Mouse IL-7 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse IL-7. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each mouse IL-7 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: E26-I154

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7

Background:

Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin8

Background: Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2

Gene ID: 16196

NCBI Accession: NP_032397

UniProt: P10168

Pathways: JAK-STAT Signaling