TFF1 ELISA Kit

Catalog No. ABIN6719950

Product Details TFF1 ELISA Kit

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: BCEI, Breast cancer estrogen inducible protein, Breast cancer estrogen inducible sequence, Breast cancer estrogen-inducible protein, D21S21, Gastrointestinal trefoil protein, Gastrointestinal trefoil protein pS2, hP1.A, HP1A, HPS 2, HPS2, pNR 2, PNR-2, pNR2, Polypeptide P1.A, Protein pS2, PS 2, pS2, pS2 protein, TFF 1, TFF1, TFF1_HUMAN, Trefoil factor 1

Background: TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Gene Name: TFF1

Production: Natural and recombinant human TFF1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: E25-F84

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TFF1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TFF1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: TFF1 (TFF1 Products)

Synonyms: BCEI ELISA Kit, D21S21 ELISA Kit, HP1.A ELISA Kit, HPS2 ELISA Kit, pNR-2 ELISA Kit, pS2 ELISA Kit, Bcei ELISA Kit, PS2 ELISA Kit, bcei ELISA Kit, d21s21 ELISA Kit, hp1.a ELISA Kit, hps2 ELISA Kit, p1 ELISA Kit, p1-a ELISA Kit, pnr-2 ELISA Kit, ps2 ELISA Kit, tff1 ELISA Kit, xP1 ELISA Kit, xp1-L ELISA Kit, xp1 ELISA Kit, TFF1 ELISA Kit, Ps2 ELISA Kit, trefoil factor 1 ELISA Kit, trefoil factor 1 S homeolog ELISA Kit, trefoil factor 1, gene 2 S homeolog ELISA Kit, TFF1 ELISA Kit, Tff1 ELISA Kit, tff1.S ELISA Kit, tff1.2.S ELISA Kit, LOC100358238 ELISA Kit

Gene ID: 7031

NCBI Accession: NP_003216

UniProt: P04155

Pathways: EGFR Signaling Pathway