Amphiregulin ELISA Kit

Catalog No. ABIN6719952

Supplier Product No.: koa0361

This Mouse Amphiregulin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Amphiregulin.

Quick Overview for Amphiregulin ELISA Kit (ABIN6719952)

Target: Amphiregulin (AREG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Amphiregulin ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0361

Supplier: Rockland

Purpose: Mouse Amphiregulin - AR Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse Amphiregulin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse Amphiregulin standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Amphiregulin standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: V100-A248

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: Amphiregulin/AR

Background:

Synonyms: A REG, AR|AREG, Colorectum cell derived growth factor, CRDGF, MGC13647, OTTHUMP00000219184, Schwannoma derived growth factor, SDGF

Background: Amphiregulin, also known as AREG, is a protein that is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to mouse chromosome 5. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. Amphiregulin is related to to epidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

Gene ID: 11839

NCBI Accession: NP_033834

UniProt: P31955

Pathways: RTK Signaling, EGFR Signaling Pathway