ADAMTS13 ELISA Kit

Catalog No. ABIN6719981

Product Details ADAMTS13 ELISA Kit

Target: ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13, A disintegrin like and metalloprotease with thrombospondin type 1 motif 13, A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13, ADAM metallopeptidase with thrombospondin type 1 motif 13, ADAM TS, ADAMTS 13, ADAMTS13, ADAMTS13 protein, ATS13_HUMAN, TTP, Von Willebrand factor cleaving protease, vWF cleaving protease, vWFCP

Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Gene Name: ADAMTS13

Production: Natural and recombinant human ADAMTS13. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: Q34-W688

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human ADAMTS13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human ADAMTS13 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for ADAMTS13

Target: ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))

Alternative Name: ADAMTS13 (ADAMTS13 Products)

Synonyms: ADAMTS13 ELISA Kit, ADAM-TS13 ELISA Kit, ADAMTS-13 ELISA Kit, C9orf8 ELISA Kit, VWFCP ELISA Kit, vWF-CP ELISA Kit, Gm710 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 13 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit, ADAMTS13 ELISA Kit, Adamts13 ELISA Kit, adamts13 ELISA Kit

Gene ID: 11093

NCBI Accession: NP_620594

UniProt: Q76LX8